SpectraMax iD5:Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. See SpectraMax® Microplate Reader User Manual Download Page. i3x. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. D 1 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. It allows you to explore cellular . The SpectraMax i3x reader provides theThe SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer can be used to image slides using a specialized slide adapter. com. 0. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. 1321N1, a human astrocytoma cell line. 3: The cuvette chamber. The SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. 흡광 (Absorbance) 리더기. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5e Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Introduction. 13. Fluorescence Microplate Reader Comparison Chart. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. Prepare your i3/i3x instrument for shipment. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. 7°C (gradient heating design). . Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signalSpectraMax i3x Multi-Mode Microplate Reader. When used in combination with the SpectraMax Injector Cartridge, this system enables a wealth of flash type luminescence applications including reporter gene and other assays with exceptional sensitivity and dynamic range. About the reader setup recommendations The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. 0´ 104 1. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. 1. 5. In addition, Image J was used to quantify the. Nucleic Acid and Protein Quantitation. Relative mitochondrial polarization was expressed as the ratio of emission at 590 nm to that at 530 nm (Shin et al. Anne T. The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Find regional support. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. com. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The FRET ratio is defined as the relative fluorescence intensity of an acceptor normalized to the total fluorescence intensity. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. The resulting absorbance was measured at 490 nm, Spectra Max i3x (Molecular Devices, USA). 0´ 104 4. The LDH activity in the cultural media was measured based on the reaction between LDH and lactic acid, which led to the generation of pyruvic acid, and pyruvic acid could become brown in alkaline environments with 2,4. 315 can't find zero order. Prepare your i3/i3x instrument for shipment. Find regional support. eu@moldev. Multimode Readers Comparison Chart. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. SCGPU05RE)A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. To learn more about the device, please watch this short video. Time-. The ratio of the two fluorescence intensities 520/620 (acceptor/donor) enables the calculation of Delta F (%) which. i3x. polarization performance of the FilterMax®, SpectraMax® Paradigm®, and select cartridges or read modes of the SpectraMax i3, i3x, and iD5 readers. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Hands-on fun and learning that empowers exploring, inventing and sharing. TUNE Tunable Wavelength Detection Cartridge. SpectraMax M 시리즈 Microplate Reader, Cuvette Port, IQ OQ 검증 | Molecular Devices. Accessories for SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . Fluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. 315 can't find zero order. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. +44 118 944 8000. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Spectranet customer portalThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 12911151) 0. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Budget-friendly multi-mode microplate reader . The dual auto injector module. The Molecular Devices SpectraMax i3x is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. The absorbance at 645 nm was subsequently determined using a Spectra Max i3x microplate reader (Molecular Devices). Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. Also refer to this Multimode Reader Comparison Chart. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Home > Search Results > Danaher Inc > spectramax i3x. Examples: fiPress carefully to engage,fl fiDo not press down. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™. The SpectraMax i3x reader provides theAbsorption was measured at 490 nm by utilizing a Spectra max i3x Multimode microplate reader (Molecular Devices). MD Neurotransmitter Transporter Assay. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellThe absorbance was recorded on a spectra Max i3x microplate reader (Molecular Devices, USA) at 450 nm. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. SeeSpectraMax MiniMaxImagingCytometeronpage85. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. 1. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Unlock your i3/i3x instrument after shipment. Accordingly, we calculated the Laurdan generalized polarization (GP) using the formula GP = (I 435 − I 490)/(I 435 +. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. SpectraMax i3x Multi-Mode Detection Platform User Guide. Microplate Readers SpectraMax. The Molecular Devices SpectraMax i3x Microplate Reader is a multi-mode microplate reader, able to measure UV/vis, fluorescence and luminescence. It allows you to explore cellular pathways and protein activation and expression in one system. 99（图一）。After incubation at room temperature for one hour in the dark, we measured the fluorescence intensity using a SpectraMax i3x (Molecular Devices, CA, USA), with excitation at 350 nm and dual emissions at 435 nm and 490 nm. Amplitude : 1. 58% for the detection module and 2. This way you’ll save time on finding the necessary info. Molecular Devicesの全商品を見る. Overview of SpectraMax MiniMax 300 Imaging Cytometer. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. SpectraMax i3x Multi-Mode Detection Platform User Guide. Figure 2-23: Bottle holder To install the bottle holder: 1. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. Find regional support. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks iD5. Data Analysis. Cet appareil ISM est confomre à la norme NMB-001 du Canada. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects and changing objectives, the i Series has you covered. 4. Explore a wealth of applications with the future-ready SpectraMax i3x. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Italic and boldface type are used for emphasis. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The chemical reduction of resazurin was evaluated incubating each well of the microtiter plate with 10 μL (10% of final well volume) of 0. 304 signal level saturation. 在SpectraMax i3x和SpectraMax iD5微孔板读板机上使用 SoftMax Pro软件绘制标准曲线，并进行4参数曲线拟合(图 4)，结果见表2。使用EC50评估该测定方法的适用性，EC50值 应低于150 nM。对于两个读板机，EC 50值均低于85 nM，处 于可接受范围内。Cisbio将可接受的信噪比定义为≥. With optimized reagents, option to operate the readers. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. 4. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,i3x Multi-Mode Microplate Reader. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 5577 Web: Email: [email protected] SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. 2. 3) At the bottom of the strip, click on "Show. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. 02, 0. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. , 2021). Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. These robust readers have been placed in labs from Antarctica to the. 2. ). Because a PMT needs to be set to a specified gain to reach a specific sensitivity,Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. pathways and protein activation and expression in one system. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Get Pricing. Meanwhile, the samples were detected with a fluorometric reader Spectra Max i3x (MD, America) at 488 nm excitation wavelength and 525 nm emission wavelength. 2 (Molecular Devices, San Jose, CA, USA). 8 Antibodies and. Multimode Reader Comparison Chart. View online or download 1 Manuals for Molecular Devices SpectraMax i3x. 200-800 nm using Spectra Max i3X microplate reader. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. Robust,. 蛍光 発光 ELISA. SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Supplier: Molecular Devices. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. The SoftMax Pro Software provides integrated instrument control. The calculated LOQ values were 0. 3. 0 mm hex key. 2. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. 304 signal level saturation. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. 305 reference level saturation. We would like to show you a description here but the site won’t allow us. The SoftMax Pro Software quickly. (SpectraMax i3/i3x) CatchPoint SimpleStep ELISA (SpectraMax Paradigm) SpectraDrop. 1. Be Future Ready with the SpectraMax i3x Multi-Mode Detection Platform. Capture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 2 only) SpectraMax M-series Microplate readers. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. 3. Simplified workflow: Add cellular imaging assays in a small footprint. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Prepare your iD series instrument for shipment. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. To read HTRF®, the SpectraMax i3x® must be first equipped with the SpectraMax i3x® Cisbio HTRF® cartridge, which enables the simultaneous measurement of both 620nm donor and 520 nm acceptor emissions. The SPECTRAmax 340PC can mea-Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Settings not required by a reader are indicated by ‘---’. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. i3x. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. 316 grating motor driver faulty. Features. 58% for the detection module and 2. Multimode Reader Comparison Chart. Pick the best speed, sensitivity, and price for your needs. Order Now. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthThe SpectraMax i3x reader grows with you as your research areas expand. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Microplate Readers and SoftMax® Pro Software. SpectraDrop Abs Protein Quant (i3-i3x)Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. Multi-Mode Reader. Instrument. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. Sunnyvale, CA, USA) with excitation at 475 nm. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. Materials and methodsWe would like to show you a description here but the site won’t allow us. 1step assay with SpectraMax microplate readers. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. 2. Modularity does provide some measure of forward vision into upcoming or future experimental needs. com The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 2. 314 low lamp level warning. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Check software compatibility with Windows 10 and locate your software installer. Find regional support. In this product video, see how the SpectraMax M5 quickly reads samples. Prime function 20 amol. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. The cell imaging was captured on Zeiss LSM 980 confocal microscopy. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Get a front row view of your cells. The SpectraMax Injector Cartridge enables precise reagent delivery and adjustment of delay and integration times that are required to obtain optimal sensitivity for this assay. ATP LLD (pM) ATP LLD (moles) SpectraMax i3x, luminescence . With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 12 mg/mL) of polysaccharides were prepared after addition of glucosum anhydricum for calibration. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. *On the SpectraMax i3x reader only. However, no piece of equipment is immune to breaking down. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. It allows you to explore cellular pathways and protein activation and expression in one system. iD5. The SpectraMax ® i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. SpectraMax® i3x 多功能读板机配置 SpectraMax® MiniMax™ 300 细胞计数 仪，具有 transmitted light (TL)，绿色 (Ex / Em: 460 / 541) 和红色 (Ex / Em: 625 / 713) 荧光通道。MiniMax 细胞计数仪的 SoftMax® Pro 软件可以用来获得各种细 胞的图像并进行分析。本说. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. Biocompare is the leading resource for up-to-date product information, product reviews, and new technologies for life scientists. Synthesis of NORP Synthesis of compound 1. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. 306 plate air cal. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. As shown in the results above, the DELFIA cell proliferation assay combined with the SpectraMax i3 reader gives researchers a very sensitive test for measuring cell proliferation. Published Dec 16, 2020 | Updated Oct 10, 2023. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthTNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. Kastelic,2 and Jian Gao 1 1Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. Prepare your i3/i3x instrument for shipment. Analysis of HTRF assays involves Cisbio’s patented ratiometric reduction method based on the two emission wavelengths detected. Danaher Inc spectramax i3x. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). i3. Spectranet customer portalPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Prismatic colour and motion for everyone. Myeloperoxidase (MPO) Assay. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. SpectraMax i3x Multi-Mode Detection Platform. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. See Unpacking the SpectraMax Paradigm Instrument on page 1. 1 mg/mL density of resazurin, protected of the light, incubated for 1 h at room temperature. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. SpectraMax Quant dsDNA Assay Kits. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes This assay workflow is easily performed using the SpectraMax i3x Multi-Mode Microplate Reader with SpectraMax Injector Cartridge, which is fully DL Ready validated. Figure 4. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. Bioz Stars score: 98/100, based on 1 PubMed citations. 2 only) SpectraMax M-series Microplate readers. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. ATP (“flash” luminescence using . Afterward, the absorbance was measured at 450 nm on a Microplate Reader (Spectra Max i3x, Molecular Devices, USA). The SPECTRAmax 340PC can mea- Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. 4. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. See5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideMolecular Interactions. We would like to show you a description here but the site won’t allow us. Find regional support. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. We would like to show you a description here but the site won’t allow us. 蛍光 発光 ELISA. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 2. Detection of pro-inflammatory factors. XPS spectra were obtained from PHI Versaprobe II. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. Structured Review. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. 2 in. SoftMax Pro. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. We would like to show you a description here but the site won’t allow us. View online or download 1 Manuals for Molecular Devices SpectraMax i3x. Promega. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Remove the transport bracket and store the retaining screws in the bag that held the 3. They were allowed to attach and grow overnight in a 37°C, 5% CO 2. Description 1. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. More Product Information. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. The fluorescence spectra was obtained on a Spectra Max i3x Multi-Mode Detection Platform. X-ray powder diffractometer (XRD) with Cu- Ka radiation (Bruker AXS D8) was used to identify the crystalline phase of as synthesized C-AuNPs. 3 In this application note we demonstrate a linear dynamic range of 6 decades for recombinant firefly and Renilla luciferases, as well as linear detection of luciferase-transfected. Description 1. 2 (Molecular Devices, San Jose, CA). china@moldev. Molecular Devices Corporation. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. ZERO BIAS - scores, article reviews, protocol conditions and more. An MPO assay has been performed to quantify the inflammation reduced by the GAR-PLGA-ES100 NPs in the inflamed cells . 吸収度マイクロプレートリーダー SpectraMax® Mini. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. StainFree Cell Detection Algorithm. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Table 1. Patented user-exchangeable cartridge design expands the system's detection capability making it. Page 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. Detection of pro-inflammatory factors. Assay setup for a 384-well low-volume plate. Register to download your free eBook today. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Microplate and read height should be optimized for each new lot of microplates or assay volume used (use the Show Pre-Read Optimization options in the instrument settings in SoftMax Pro Software). Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. It allows you to explore cellular pathways and protein activation and expression in one system. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. Explore a wealth of applications with the future-ready SpectraMax i3x. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. Use the 3.