*On the SpectraMax i3x reader only. Molecular Devices are known for both the reliability and the wide uses of their products. Spectranet customer portal The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Learn more about its features, specifications, and applications in this user guide. QBT Fatty Acid Transporter Assay. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The UV-Vis spectroscopy was measured on a TU-1900Beijing PuXi instrument. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. 1. The SPECTRAmax 340PC can mea-Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. Prismatic colour and motion for everyone. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. *On the SpectraMax i3x reader only. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. These robust readers have been placed in labs from Antarctica to the. Description 1. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. pathways and protein activation and expression in one system. eu@moldev. Donor emission at 616 nm is used as an internal reference, while acceptor emission at 665 nm. Remove the transport bracket and store the retaining screws in the bag that held the 3. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks iD5. 99（图一）。After incubation at room temperature for one hour in the dark, we measured the fluorescence intensity using a SpectraMax i3x (Molecular Devices, CA, USA), with excitation at 350 nm and dual emissions at 435 nm and 490 nm. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strength*On the SpectraMax i3x reader only. All test measurements and calculations are handlediD5. The difference in absorbance observed in the presence and absence of SOD was considered to be the amount of superoxide produced, and results. We would like to show you a description here but the site won’t allow us. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Myeloperoxidase (MPO) Assay. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. i3. 3 In this application note we demonstrate a linear dynamic range of 6 decades for recombinant firefly and Renilla luciferases, as well as linear detection of luciferase-transfected. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. The standard solutions (0. 6% for the on-board optics (monochromators). 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. SpectraMax i3x Multi-Mode Microplate Reader System. Unlock your i3/i3x instrument after shipment. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. , 2021). Register to download your free eBook today. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. As shown in the results above, the DELFIA cell proliferation assay combined with the SpectraMax i3 reader gives researchers a very sensitive test for measuring cell proliferation. 2. Carbonyl assay The plates for carbonyl detection were prepared as described. Supplier: Molecular Devices. Analysis of HTRF assays involves Cisbio’s patented ratiometric reduction method based on the two emission wavelengths detected. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The corrected absorbance is within 5% of the. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. AquaMax Microplate Washer. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. The turbidity. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. This way you’ll save time on finding the necessary info. 58% for the detection module and 2. Kastelic,2 and Jian Gao 1 1Department of Clinical Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China. SpectraMax i3x读板机针对96孔板和384孔板进行检测时，最低检测下限可至10细胞每孔。 此种检测方法的灵敏度远远高于标准比色法和绝大多数荧光法。 CellTiter-Glo试剂检测线性范围覆盖所有细胞浓度区间，动态学检测范围大于3个数量级且r2 > 0. Detection of pro-inflammatory factors. An MPO assay has been performed to quantify the inflammation reduced by the GAR-PLGA-ES100 NPs in the inflamed cells . Figure 3. It allows you to explore cellular pathways and protein activation and expression in one system. com. Patented user-exchangeable cartridge design expands the system's detection capability making it. 1321N1, a human astrocytoma cell line. The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. The chamber. Microplate reader assays requiring infrared (IR) or red-shifted. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Meanwhile, the samples were detected with a fluorometric reader Spectra Max i3x (MD, America) at 488 nm excitation wavelength and 525 nm emission wavelength. at 540 nm using a Spectra Max i3x microplate reader (Molecular devices, San Jose, CA). Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Features. Actual shake speed is based on the microplate format. 3. 5 µL anti-cAMP-Cryptate. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformNORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 02, 0. Downloads. Price from $9. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. D. The chamber. Prime function 20 amol. 314 low lamp level warning. Spectranet customer portalSpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. The intensity of the emitted light can be measured easily with the SpectraMax i3x reader. Molecular Devicesの全商品を見る. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. A: percent GFP-positive cells calculated using the classification feature in the software. 0 | 9 reviews | Write your own review Measures Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgradesMolecular Devices SpectraMax i3x Multi-Mode Microplate Platform. 0´ 104 1. The information below applies to a standard 96 well plate, set to a linear shake mode. 2. polarization performance of the FilterMax®, SpectraMax® Paradigm®, and select cartridges or read modes of the SpectraMax i3, i3x, and iD5 readers. SpectraMax i3/i3x readers must be equipped with a suitableWhen we measured the FRET ratio using the Spectramax i3x plate reader, we found that FRET ratios were higher in DMSO-treated cells that had not been incubated at room temperature to enhance fluorescence than in room temperature-incubated cells (Fig. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology*On the SpectraMax i3x reader only. ). 5577 Web: Email: [email protected] SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. D 1 1. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. Relative mitochondrial polarization was expressed as the ratio of emission at 590 nm to that at 530 nm (Shin et al. Spectranet customer portalWatch this video to get an introduction to the Injector-Ready SpectraMax® i3x Multi-Mode Microplate Reader System. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Contact Local Team. Absorbance: For wavelengths in the visible range, 340 nm and above, clear polystyrene plates are recommended. comThe SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. Spectranet customer portal The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. i3. Supplier Page. We would like to show you a description here but the site won’t allow us. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. SpectraMax ® iD3 and iD5 Multi-Mode . pathways and protein activation and expression in one system. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 312 gain calibration failed. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using a Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA, USA) with the excitation and emission setup provided with SoftMax Pro 7. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. Spectranet customer portalPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. To learn more about the device, please watch this short video. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. Multimode Reader Comparison Chart. 3) At the bottom of the strip, click on "Show. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. Published Dec 16, 2020 | Updated Dec 16, 2020. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Besides, it’s possible to examine each page of the guide singly by using the scroll bar. 제품. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. 1) Select the correct plate and the wells that you want to measure. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. The fluorescence quantum yield (QY) of the Pdots was measured by a UV−NIR absolute PL QY. In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. Accessories for SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. , 2021). The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Explore a wealth of applications in one future-ready system that measures spectral-based absorbance, fluorescence, and luminescence. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Multimode Readers Comparison Chart. Fluorescence Microplate Reader Comparison Chart. 価格、見積もり、お近くの販売店を. It also integrates with the. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Next,. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Find regional support. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. , 2011). Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. Sensitive TRF detection of two simple, mix-and-measure assay workflows. SpectraMax® i3x 多功能读板机配置 SpectraMax® MiniMax™ 300 细胞计数 仪，具有 transmitted light (TL)，绿色 (Ex / Em: 460 / 541) 和红色 (Ex / Em: 625 / 713) 荧光通道。MiniMax 细胞计数仪的 SoftMax® Pro 软件可以用来获得各种细 胞的图像并进行分析。本说. 2 (Molecular Devices, San Jose, CA, USA). Bioz Stars score: 98/100, based on 1 PubMed citations. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. HeLa cells were plated at 15,000 cells per well in 100 µL of medium in a 96-well black, clear-bottom microplate. Fluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection. 306 plate air cal. See Unpacking the SpectraMax Paradigm Instrument on page 1. Spectranet customer portalThe SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. Fluorescence Reader. Capture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. High resolution X-ray photoelectron spectroscopy (XPS) of cCQD was obtained on a K-Alpha+ (Thermo Fisher Scientific, Massachusetts, USA) spectrometer. The SpectraMax ® i3x reader provides the. However, no piece of equipment is immune to breaking down. ) wide and 65 cm (25. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. ISM Equipment Classification (Group 1, Class A) This equipment is. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. +44 118 944 8000. Increased fluorescence performance. Table 1. detection cartridge LUM96: 59. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataThe SpectraMax i3x reader and MiniMax cytometer can be used with FluoroBlok cell culture inserts to directly count cells from high-quality images to measure cellular migration over time. The SpectraMax i3x reader provides the With these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. Get a front row view of your cells. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. 00800 665 32860. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 5. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. This way you’ll save time on finding the necessary info. 19 Switch on the SpectraMax i3x plate reader and launch SoftMax Pro software. 吸収度マイクロプレートリーダー SpectraMax® Mini. Explore a wealth of applications with the future-ready SpectraMax i3x. iD5. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Key features. i3x. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). 316 grating motor driver faulty. To detect physical stability, Pdots were treated with water and incubated with plants. Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an. spectramax i3x (Danaher Inc)The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. SpectraMax i3x Multi-Mode Microplate Reader SystemSpectraMax® i3x dari Molecular Devices adalah sistem deteksi berbasis multi-mode yang berevolusi menyesuaik. In addition, Image J was used to quantify the. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Assay setup for a 384-well low-volume plate. Fluorescence emission spectra were collected on an ELISA molecular system (Spectra Max i3x). 58% for the detection module and 2. How to properly unlock iD series instrument. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks. It allows you to explore cellular . • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. com The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Read height should be optimized for . , Fisher Scienti fi c, cat. Time-. Image analysis provides accurate data. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. com. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. SpectraMax iD5:Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 1. Spectramax I3x, supplied by Danaher Inc, used in various techniques. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. Multimode Reader Comparison Chart. Products : SoftMax Pro Software, SoftMax Pro GxP Software, CellReporterXpress software, General, Screenworks, pCLAMP/AxoScope, GenePix Pro, MetaXpress software. Figure 4. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Turbidity measurements were performed with a Spectra Max® i3x microplate reader to study the effect of oxidation on the kinetics of sFN formation. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Expand your lab’s capabilities to include flash applications such as dual luciferase and. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. HTRF IP standard curve. Be Future Ready with the SpectraMax i3x Multi-Mode Detection Platform. Table 1. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. 0 mm hex key. Robust,. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. Wokingham, Berkshire RG41 5TS United Kingdom. It can be upgraded with modules such as imaging cytometer, western blot imager, and injectors to expand its capabilities. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax MiniMax 300 イメージングサイトメーター は、研究室でのアップグレードが可能なSpectraMax i3xシステム専用のオプションで、ユーザーは複雑なイメージングシステムに投資することなく、細胞増殖を解析できます。SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. 7°C (gradient heating design). Specifically, we present neurite. See more, and do more, with the SpectraMax® i3/i3x Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick imaging and analysis of cells and gives you a front-row view of phenotypic changes that accompany cytotoxicity,. 0´ 105 dsD NA (p g/ wel ) R F USpectraMax Glo Steady-Luc 工作液都加 入到微孔板后，震荡摇匀并避光放置 10 min。使用 SpectraMax Glo Steady-Luc Reporter 内置的 SpectraMax Glo Steady- Luc Reporter 实验模板设定程序并通过 SpectraMax i3x 读板机检测发光。检测结 束后，模板会根据数据自动生成一条曲 线。SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. The SpectraMax Injector Cartridge enables precise reagent delivery and adjustment of delay and integration times that are required to obtain optimal sensitivity for this assay. The plate was shaken and incubated in the dark for 10 minutes. SoftMax Pro GxP Compliance Software. Settings not required by a reader are indicated by ‘---’. Nucleic Acid and Protein Quantitation. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. The SoftMax Pro Software provides integrated instrument control. 0. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Italic and boldface type are used for emphasis. 12911151) 0. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Multi-Mode Reader. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. , 1997). Microplate Reader. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. SpectraMax i3x Multi-Mode Detection Platform User Guide. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Register to download your free eBook today. 3: The cuvette chamber. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. Note: Additional readers with similar performance are listed. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Introduction. ，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. The resulting absorbance was measured at 490 nm, Spectra Max i3x (Molecular Devices, USA). SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. The dynamic range of the assay is about 3. Fluorescent Polarization: Solid black plates, as above. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. Find regional support. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. However, no piece of equipment is immune to breaking down. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Multimode Readers Comparison Chart. Dependingontheapplication,theinstrumentcanread6,12,24,48,96,and384-well microplates. The SpectraMax i3x from Molecular Devices is a multi-mode plate reader that can measure spectral-based absorbence and luminescence. 1 mg/mL density of resazurin, protected of the light, incubated for 1 h at room temperature. VAL014, right table) assays on the SpectraMax i3x reader are shown below. 0´ 104 6. The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. Download PDF. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 2. Ease of use. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. 303 unable to cal dark current. SpectraMax i3, i3x, and iD5 readers. 2C). 2 only) SpectraMax M-series Microplate readers. It allows you to explore cellular pathways and protein activation and expression in one system. 7 mm for all shake intensities. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. Briefly, after exposed to ZnO/GO and Cd, cells were collected and washed with ice-cold PBS twice and then re-suspended in 200 μL binding buffer. SCGPU05RE)A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Learn how the SpectraMax Paradigm Multi-Mode Detection Platform with Tunable Wavelength (TUNE) Detection Cartridge combines the flexibility of wavelength scanning with the high sensitivity of filters. flValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 2. techsupport. Description 1. The absorbance at 450 nm was read with a Spectra Max i3X microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA), and the data at 30–40 min post-treatment were analyzed. Order Now. 1. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Prepare your i3/i3x instrument for shipment. See SpectraMax® Microplate Reader User Manual Download Page. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. It allows you to explore cellular pathways and protein activation and expression in one system. SpectraMax Microplate Readers. The absorbance at 645 nm was subsequently determined using a Spectra Max i3x microplate reader (Molecular Devices). 304 signal level saturation. The device can be upgraded to include capabilities for Western blotting, imaging and injection. Increased fluorescence performance. Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signalSpectraMax i3x Multi-Mode Microplate Reader. . Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. Both Microplate Optimization and Read Height Adjustment were. Also refer to this Multimode Reader Comparison Chart. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. 2. Thetypeof luciferase(Renilla. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. Prepare your iD series instrument for shipment. 1 1 10 100 e e e e e e eThe SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Increased fluorescence performance. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Molecular Devices are known for both the reliability and the wide uses of their products. Shake intensity.