在SpectraMax i3x和SpectraMax iD5微孔板读板机上使用 SoftMax Pro软件绘制标准曲线，并进行4参数曲线拟合(图 4)，结果见表2。使用EC50评估该测定方法的适用性，EC50值 应低于150 nM。对于两个读板机，EC 50值均低于85 nM，处 于可接受范围内。Cisbio将可接受的信噪比定义为≥. Fluorescence emission spectra were collected on an ELISA molecular system (Spectra Max i3x). Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. eu@moldev. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12，相关视频：多功能酶标仪SpectraMax i3x的维护操作-注射器卡盒，多功能酶标仪SpectraMax iD5的维护操作-注射器卡盒，多功能酶标仪SpectraMax i3x的维护操作-卡盒的安装和拆卸，细胞计数及活率分析仪 Vi-CELL BLU 功能&操作讲解，CRISPR基因编辑系统介绍：Cas9和Cas12a，流式. , 1997). The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. 4. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). Open the catalog to page 1. Prime function 20 amol. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Multimode Reader Comparison Chart. , 2011). com Check our website for a current listing of worldwide distributors. X-ray powder diffractometer (XRD) with Cu- Ka radiation (Bruker AXS D8) was used to identify the crystalline phase of as synthesized C-AuNPs. Detection of pro-inflammatory factors. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. This way you’ll save time on finding the necessary info. Data Analysis. Molecular Devices Software. ) deep, preferably with access on all sides. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Microplate Readers and SoftMax® Pro Software. SpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. TUNE Tunable Wavelength Detection Cartridge. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Pick the best speed, sensitivity, and price for your needs. 吸収度マイクロプレートリーダー SpectraMax® Mini. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. To read HTRF®, the SpectraMax i3x® must be first equipped with the SpectraMax i3x® Cisbio HTRF® cartridge, which enables the simultaneous measurement of both 620nm donor and 520 nm acceptor emissions. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. Cet appareil ISM est confomre à la norme NMB-001 du Canada. ATP standard curves read on SpectraMax microplate readers. Bioz Stars score: 98/100, based on 1 PubMed citations. Devices instruments including: SpectraMax®, VersaMax, VMax®, EMax®, Gemini, ThermoMax, and FlexStation® readers. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Manufacturers try to design their instruments so they can be. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax MiniMax 300 イメージングサイトメーター は、研究室でのアップグレードが可能なSpectraMax i3xシステム専用のオプションで、ユーザーは複雑なイメージングシステムに投資することなく、細胞増殖を解析できます。SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. Bioz Stars score: 98/100, based on 1 PubMed citations. SpectraMax Quant dsDNA Assay Kits. 1. More Product Information. Its dual light sources, a xenon lamp and LEDs, illuminates samples with incomparable signal strength and superior sensitivity. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. Fluorescence Microplate Reader Comparison Chart. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5e Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. 0. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. The device can be upgraded to include capabilities for Western blotting, imaging and injection. The calculated LOQ values were 0. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. 200-800 nm using Spectra Max i3X microplate reader. Fluorescence was measured using a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA) with 365 nm excitation and 450 nm emission filters. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The turbidity. 吸収度マイクロプレートリーダー SpectraMax® Mini. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. The resulting absorbance was measured at 490 nm, Spectra Max i3x (Molecular Devices, USA). DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. It allows you to explore cellular pathways and protein activation and expression in one system. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely low light. SpectraMax i3x 一套系统即可以帮助你通过科学手段解开细胞通路的奥秘，也可以检测并分析出 样品中蛋白质的活性和表达情况。SpectraMax i3x 检测系统可以根据未来实验需求，无需售后工 程师协助即可加入全新检测功能模块，真正做到一次投入终身受益。1) Select the correct plate and the wells that you want to measure. ) wide and 65 cm (25. pathways and protein activation and expression in one system. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. flValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. User-upgradeable detection modules allow you to expand the capabilities of your microp. 303 unable to cal dark current. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. We would like to show you a description here but the site won’t allow us. Generate more data points without the need to dilute. ISM Equipment Classification (Group 1, Class A) This equipment is. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Enzyme Activation Analysis;. The corrected absorbance is within 5% of the. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. 모든 제품. , 2014; Xiong et al. The calculated LOQ values were 0. It allows you to explore cellular . Page 9 Setting Up the SpectraMax® MiniMax™ 300 Imaging Cytometer 2. com. i3x. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Average 98 stars, based on 1 article reviews. 316 grating motor driver faulty. SpectraMax i3x Multi-Mode Detection Platform User Guide. It allows you to explore cellular . KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologySpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Figure 4. The Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). Description 1. The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. 800. flThe SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. How to properly unlock iD series instrument. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformNORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. It allows you to explore cellular pathways and protein activation and expression in one system. 312 gain calibration failed. 99 to $1999. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. +44 118 944 8000. SpectraMax iD5:Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. UV-Vis absorbance, fluorescence and luminescence • Accommodates plate types from six to 384-well format • Easily upgradeable with interchangeable filter cubesSpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Introduction. Briefly, after exposed to ZnO/GO and Cd, cells were collected and washed with ice-cold PBS twice and then re-suspended in 200 μL binding buffer. 303 unable to cal dark current. The plate was shaken and incubated in the dark for 10 minutes. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. No Stain Analysis: Eliminate cell staining for cell counting and confluence measurements using. The dual auto injector module. The assay window spanned four orders of magnitude. See SpectraMax® Microplate Reader User Manual Download Page. Prepare your iD series instrument for shipment. Description 1. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. The intensity of the emitted light can be measured easily with the SpectraMax i3x reader. SpectraMax M 시리즈 Microplate Reader, Cuvette Port, IQ OQ 검증 | Molecular Devices. Every SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. 1) Select the correct plate and the wells that you want to measure. 2. The corrected absorbance is within 5% of the value. A: percent GFP-positive cells calculated using the classification feature in the software. 1 mg/mL density of resazurin, protected of the light, incubated for 1 h at room temperature. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). Unlock your i3/i3x instrument after shipment. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Figure 1. SpectraMax i3x Multi-Mode Microplate Reader SystemSpectraMax® i3x dari Molecular Devices adalah sistem deteksi berbasis multi-mode yang berevolusi menyesuaik. 1. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Table 1. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. 13. Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. Time-. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. To detect physical stability, Pdots were treated with water and incubated with plants. 0´ 104 6. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. Examples: “Press carefully to engage,” “ Do not. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. 00800 665 32860. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Animal imaging assay was performed on a IVIS R Lumina Series III small animal optical system. For aqueous solutions, our patented* PathCheck® sensor technology senses the depth of the liquid in each microplate well, and normalizes the absorbance value to a 1-cm pathlength. Spectramax I3x, supplied by Danaher Inc, used in various techniques. Multi-Mode Reader. We would like to show you a description here but the site won’t allow us. The soil suspension (600 µL) and the substrate (L-3,4-dihydroxyphenylalanine, DOPA, 150 uL, 25 mmol L -1 ), were mixed and then added to. In this product video, see how the SpectraMax M5 quickly reads samples. i3. SpectraMax i3x. 08, 0. About the reader setup recommendations The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Carbonyl assay The plates for carbonyl detection were prepared as described. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. SpectraMax® i3x Multi-Mode Detection Platform, Molecular Devices. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Bioz Stars score: 98/100, based on 1 PubMed citations. Example: [Drawer]. Multimode Reader Comparison Chart. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. The SpectraMax i2X is used for cell migration, cell. Bookings are free, but you will need to use iLab to book. Description 1. Ferguson, Ph. Table 3 Optimal instrument settings for Valita Titer Plus assay Fluorescence Polarization measurement on Molecular Devices microplate readers. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Both Microplate Optimization and Read Height Adjustment were. Check software compatibility with Windows 10 and locate your software installer. The plate was shaken and incubated in the dark for 10 minutes. Table 1. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. 2. 315 can't find zero order. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Nucleic Acid and Protein Quantitation. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. In this application note, we describe how the SpectraMax ® i3x Multi-Mode Microplate Reader with SpectraMax Injector Module is used to perform calcium assays on two different cell lines. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the datathe SpectraMax i3x Multi-Mode Microplate Reader with SpectraMax Injector Cartridge Introduction Reporter gene assays are used to study the expression of eukaryotic genes. Danaher Inc spectramax i3x. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. Cell counters for accurate and. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. Relative mitochondrial polarization was expressed as the ratio of emission at 590 nm to that at 530 nm (Shin et al. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Sensitive TRF detection of two simple, mix-and-measure assay workflows. Molecular Devices Corporation. 315 can't find zero order. Thetypeof luciferase(Renilla. However, no piece of equipment is immune to breaking down. Find regional support. However, no piece of equipment is immune to breaking down. 22 μ m fi ltration unit (Sigma, cat. • Removing the transport locks from the PMT shutters and drawers. Prepare your i3/i3x instrument for shipment. Find regional support. 1. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. When the user applies a set temperature of 37°C, the top is heated to 37. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. D 1 1. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. MTT was purchased from Sigma-Aldrich (St. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. each new assay. SpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. The chamber. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added. i3. Promega. The LDH activity in the cultural media was measured based on the reaction between LDH and lactic acid, which led to the generation of pyruvic acid, and pyruvic acid could become brown in alkaline environments with 2,4. • SpectraMax i3x • Onboard luminescence • Detection cartridge (LUM 96) • SpectraMax iD5 • SpectraMax L • SpectraMax M5e • SpectraMax Mini. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SoftMax Pro Software provides integrated instrument control. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,i3x Multi-Mode Microplate Reader. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . Microplate Readers SpectraMax. 0. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,*On the SpectraMax i3x reader only. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . It allows you to explore cellular pathways and protein activation and expression in one system. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. 0´ 104 8. 2. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the. Formicro-volumemeasurements,theinstrumentsupportsSpectraDrop24-wellSpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. 0´ 105 dsD NA (p g/ wel ) R F USpectraMax Glo Steady-Luc 工作液都加 入到微孔板后，震荡摇匀并避光放置 10 min。使用 SpectraMax Glo Steady-Luc Reporter 内置的 SpectraMax Glo Steady- Luc Reporter 实验模板设定程序并通过 SpectraMax i3x 读板机检测发光。检测结 束后，模板会根据数据自动生成一条曲 线。SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. 304 signal level saturation. HeLa cells were plated at 15,000 cells per well in 100 µL of medium in a 96-well black, clear-bottom microplate. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. HTRF IP standard curve. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. 2 (Molecular Devices, San Jose, CA, USA). Red and green fluorescence was then measured (emission at 590 and 530 nm, respectively, with excitation at 490 nm) using a fluorescence microplate reader (Spectra Max i3x, Molecular Devices Inc. Example: [Drawer]. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Native or oxidized AN (40 μM) and FN (0. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. 4. Fluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). Molecular Devices are known for both the reliability and the wide uses of their products. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. 2. *On the SpectraMax i3x reader only. Absorbance was measured on the SpectraMax i3x Multi-Mode Platform at 475 nm and 660 nm after 1-4 hours of incubation. SCGPU05RE)A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Videos & Webinars. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. D. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. 99（图一）。The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Videos & Webinars SpectraMax i3x - Detection Module ChangeSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Description 1. 9 x 10-15. applications, including dual luciferase and ATP assays. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. It allows the user to determine the total concentration of protein in a solution. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. i3x. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. detection cartridge LUM96: 59. Simplified workflow: Add cellular imaging assays in a small footprint. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. polarization performance of the FilterMax®, SpectraMax® Paradigm®, and select cartridges or read modes of the SpectraMax i3, i3x, and iD5 readers. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Ease of use. The TRF intensities measured are proportional to the level of extracellular acidification in each sample. As shown in the results above, the DELFIA cell proliferation assay combined with the SpectraMax i3 reader gives researchers a very sensitive test for measuring cell proliferation. Microplate Reader. Winnersh Triangle. Get a front row view of your cells. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. Capture Flash Assays with Ease. GFP expression imaged on the cytometer. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. Read height should be optimized for . Explore a wealth of applications with the future-ready SpectraMax i3x. 306 plate air cal. Assay setup for a 384-well low-volume plate. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Key features. 4. SoftMax Pro Software enables users to quickly set up analysis of cell counts via a simple point and click user interface. For wavelengths in the UV range above 220 nm,. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. Table 1. Prepare your i3/i3x instrument for shipment. 58% for the detection module and 2. 2/5 apart, not needed o Otherwise, ensure cutoff is 10nm less than desired emission wavelength. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. Nuclear Receptor. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects and changing objectives, the i Series has you covered. Myeloperoxidase (MPO) Assay. 0´ 104 1. After correcting for negative controls and quench standards, the soil enzyme activities were expressed in units of nmol h −1 g −1 soil (German et al. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Features. See Unpacking the SpectraMax Paradigm Instrument on page 1. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. #0200-7009) The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 蛍光 発光 ELISA. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. Detection cartridge modularity allows for easy addition of new read modes within minutes without. Published Dec 16, 2020 | Updated Dec 16, 2020. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. An MPO assay has been performed to quantify the inflammation reduced by the GAR-PLGA-ES100 NPs in the inflamed cells . SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataBased on the SpectraMax i3x system, ACROBiosystems provides biomedical research and development services for customers seeking quantification and detection of antibodies-antigen interaction. Next,. Prepare your i3/i3x instrument for shipment. 7 mm for all shake intensities. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The user guide also covers the features, specifications, and software of the reader, as well as the installation and. Description 1.