Table 2 summarizes results of the Quant-iT Prepare the first standard Combine 20uL of 100ug/mL lambda DNA from the Quant-IT PicoGreen dsDNA assay kit (Fisher P7589) with 980uL 1X TE to create a 2ug/mL standard. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Picogreen assays are compatible with most microplate readers and fluorometers. Quant It. 0 fluorometer according to the manufacturer’s protocols; a. Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. We use the Quant-iT™ PicoGreen® dsDNAkit for quantification of both extracted and amplified DNA. 2–100 ng: 2-1,000 ng: 0. 50 pg to 2 µg. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT PicoGreen dsDNA Assay Kit 를 사용하면, ssDNA 와 RNA 그리고 free nucleotide 가 혼재되어 있는 시료에서도 dsDNA 만을 선택적으로 측정할 수 있으며, 25 pg /ml 의 매우 적은 양까지도 검출할 수 있습니다. 86100 stars. High tolerance for contaminants. In addition to conventional determination using 5 standards as present-ed by the manufacturer, we will show that reliable determi-nation is possible using only 2 standards. 线，利用这一曲线并根据经验检测样本的浓度。Qubit dsDNA HS Assay可用于测量DNA浓度在10pg/µL 至100ng/µL 之间 的样本。超过此浓度范围的样本必须经过稀释然后进行重新 测试，以确保它们的结果落在参考标准的线性范围内。Qubit dsDNA HS Assay具有高度的准确. PicoGreen dsDNA quantitation reagents are. Rapidly detect as little as 0. (An additional 1 ml TE with 5 ul PicoGreen may need to be prepared for the standards. The sample must yield a minimum of 55 ng of genomic DNA to ensure sufficient DNA for quality control (QC) and. Average 86 stars, based on 1. 5 ng/ml ~ 1,000 ng/ml 까지 정비례. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Quantitation Range. A 96 plate well was filled with 100 µl of dye and 100 µl of DNA, a 384 well with 10 µl of each solution. PicoGreen dsDNA quantitation reagents are. 0 fluorometer (Selection Guide for the Qubit and Quant-iT Assay Kits. 2. 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. 25 pg/uL 的 dsDNA。 该定量试剂盒在三个数量级内呈线性，几乎无序列依赖性，可让您准确测定多种来源的 DNA，包括基因组 DNA、病毒 DNA、小提 DNA 或 PCR 扩. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of ssDNA and RNA with minimal effect on the quantitation results. 50 pg to 2 µg. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 0 ml 200x in DMSO b. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesIn this research six cyanine fluorophores for the quantification of dsDNA in the pg-ng range, without amplification, are compared under exactly identical conditions: EvaGreen, SYBR Green, PicoGreen, AccuClear, AccuBlue NextGen and YOYO-1. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 2023. The x-axis gives the mass of nucleic acid at a given point when DNA or RNA is assayed alone. For a standard. 3. As little as 2. Molecular Probes fluorescent and luminescent protein blot stains—Table 9. ZERO BIAS - scores, article reviews, protocol conditions and moreGot it. no. 5), 200X PicoGreen. Serial dilutions were made to give appropriate cell, seeding numbers. Tweet. Rapidly detect as little as 0. g. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Picogreen assays are compatible with most microplate readers and fluorometers. ) Add PicoGreen to DNA samples Add 100 1x PicoGreen to each well of the DNA/standard plate without contaminating tips. Triplicate 10 μL samples of λ DNA (Ο), E. B–F are steps of the assay plate preparation using an test1. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Picogreen kinetic assay was based on polymerase activity of SARS nsp12 in complex with nsp7L8, which. using a PicoGreen assay by the company life technologies (Quant-iT™ PicoGreen dsDNA Reagent and Kits). 0 mL to 200 uL to fit a 96-well microplate format. The Qubit dsDNA HS and BR Assay Kits are accurate from 10 pg/L to 100 ng/uL, and 100 pg/L to 1,000 ng/L, respectively. Quant-iT™ PicoGreen® dsDNA Assay KitLife TechnologiesCatalog #P7589 protocols. ZERO BIAS - scores, article reviews, protocol conditions and moreComparison of dsDNA dose-response using the Helixyte Green™ Fluorimetric dsDNA Quantitation Kit *High Sensitivity* (blue) with Quant-iT™ PicoGreen ® dsDNA Assay Kit (red ). Q33130 Introduction The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and. 10 x 100 µL. Supplier: Invitrogen™ P11496. Rapidly detect as little as 0. The Quant-iT™ OliGreen™ ssDNA Assay Reagent enables researchers to quantify as little as 1 ng/mL oligonucleotide or ssDNA (200 pg in a 200 µL assay volume) with a. 99. The kit is robust with a mix and read format compatible with 96- and 384-well fluorescence-based microplate readers. • Suitable for use in 96- and 384-well plate formats. The AccuBlue® Broad Range RNA Quantitation Kit is highly selective for RNA over dsDNA. 1 Prepare a 2 μ g/mL stock solution of dsDNA in TE. The Quant-iT PicoGreen dsDNA. If you would like to use this kit with the Qubit® fluorometer, we have includedQuant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. This protocol is a simplified and condensed version of. Measure concentration with a high-sensitivity assay (e. 2. 100 ng/µL of Lamba (λ) DNA, sheared by intensive. NET formation quantification was performed using a MPO-DNA PicoGreen assay according to the manufacturer’s instructions (Quant-iT PicoGreen dsDNA Assay kit, Invitrogen). The stock DNA and the corresponding 1:5 diluted DNA derived from FFPE tissue was quantified by using Quant-iT™ PicoGreen dsDNA Assay kit (Thermo Fisher Scientific) as follows: 2 μl of sample DNA were diluted 1:5 vol/vol in 1× TE, containing 1:100 diluted PicoGreen reagent in a final reaction volume of 40 μl per reaction. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and the Quant-iT Broad-Range dsDNA Assay Kit provide concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. 1–100 ng: Ex / Em (nm) 502 / 523: 510 / 527: 502 / 523: Components: dsDNA HS fluorescent reagent, dilution buffer and standards RCA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. Catalog Number Specifications Unit Size Product Type Quantitation Range Packaging Type Price (EUR) Availability Quantity;The assay was prepared for 184 aliquots of DNA sample and five DNA standards, including one blank. dsDNA in cytoplasmic fractions was quantified using the Quant-iT PicoGreen dsDNA. A comparison of reagents for detecting and quantitating proteins in solution—Table 9. Bioz Stars score: 86/100, based on 1 PubMed citations. 2009;49(4):997–1002. Quant-iT PicoGreen dsDNA Assay Kits and Reagents are designed to selectively detect dsDNA from a wide variety of sources, even in presence of ssDNA, RNA, and free. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). Both fluorescent assays were performed with a Qubit fluorometer. Picogreen assays are compatible with most microplate readers and fluorometers. Rapidly detect as little as 0. Similar. Highly selective for dsDNA over ssDNA or RNA. PicoGreen dsDNA quantitation reagents are. Quant-iT™ PicoGreen® dsDNA Assay Kit *10 x 100 µl* 1 kit P11496 Quant-iT™ PicoGreen® dsDNA Reagent 1 kit P7581 Quant-iT™ PicoGreen® dsDNA Reagent *10 x 100 µl* 1 kit P11495 Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 1 kit R11491 Quant-iT™ RiboGreen® RNA Assay Kit *2,000 assays* 1 kit R11490 Quant-iT™ OliGreen™ ssDNA. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). No. Materials: 1. The Quant-iT™ PicoGreen® DNA assay is the oldest assay and the most general-purpose. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM製品番号（カタログ番号）： P11495. Analysis of cfDNA and H3Cit was performed using Quant-iT™ PicoGreen ® dsDNA Assay Kit or an ELISA, respectively. Quantitation Range. Before using PicoGreen dye to stain DNA, the concentrated Me 2 SO solution containing PicoGreen dye was diluted 200-fold using 1× TE buffer to prepare 2× PicoGreen working solution. Buy from Supplier. 产品概述. The fluorescence spectra of dsDNA stained with PicoGreen when in solution equimolar concentration of RNA was also present ([DNA]/[RNA] = 1, red lines in figure 4) did notObtenga mediciones precisas de la concentración de ADNbc en un amplio rango dinámico con los kits de ensayo de ADNbc y reactivos de ADNbc Quant-iT Picogreen. 2023. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). It is specific for dsDNA in the presence of equimolar amounts of RNA. A12216) Metabolic (glucose) • Amplex Red Glucose/Glucose Oxidase Assay Kit (Cat. 99 to $1999. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Rapidly detect as little as 0. For example, if the assay requires 50 wells, add 600 L of PicoGreen to 14. Quant-iT™ PicoGreen™ dsDNA Assay KitInvitrogen - Thermo FisherCatalog #P11496 Quant-iT™PicoGreen® dsDNA reagent is dissolved in dimethylsulfoxide (DMSO), which freezes below 19 °C. 2 The input amount of linear dsDNA library is based on the available linear dsDNA (see Table 3). PicoGreen dsDNA quantitation reagents are. Skip to main list 美谷分子仪器（上海）有限公司. (Waltham, MA, United States) using the Quant-iT TM PicoGreen TM dsDNA Assay Kit. 使用 PicoGreen dsDNA 定量试剂盒和试剂时，即使在存在 ssDNA、RNA 和游离核苷酸的情况下，也可快速检测低至 0. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. Description. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). For running these libraries on the MiSeq and HiSeq, please make sure you read the supplementary methods of Caporaso et al. It must be completely liquified before use by allowing it to come to room temperature. low-range. 0 ml) of the aqueous working solution of Quant-iT™ PicoGreen dsDNA Reagent was added to each cell sample, which was incubated for 10 min at 37 °C in the dark. Materials & Equipment Required But Not Provided • Pipettors - 5 L - 1200 L. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. a) 16. Quantification and normalization of double stranded DNA with minimal set-up time. Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. Libraries were quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies) and the Kapa Illumina GA with Revised Primers-SYBR Fast Universal kit (Kapa Biosystems). Protocols All Picogreen reactions and measurements werethe Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. 1. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. このアッセイは、従来のUV吸光度法に比べて感度が何倍も高いため、様々な濃度範囲に対して最適化することが可能です。. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). The following protocol is an adaptation of the Mo-lecular ProbesTM dsDNA Quantitation Reagent and Kits product information sheet. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile,Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). The kit contains. Q33120). 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 5. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The AccuBlue® High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with a linear detection range from 0. 86 / 100 stars. 8 Measurement of growth factor content in. dsDNA in cytoplasmic fractions was quantified using the Quant-iT PicoGreen dsDNA. In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Easy to use UV absorbance alternative assay that can be. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and. Description. The accuracy index is defined in Table 2. The Quant-iT™ dsDNA BR assay can be adapted for use in cuvettes or 384-well microplates. the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. 3. Quantitation using Quant-iT Assay Kits. 5 µL of each extracted sample was used for Picogreen analysis. Quantitation Range. Final libraries were quantified using a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on a SpectraMax iD3 (Molecular Devices). P7589. Picogreen assays are compatible with most microplate readers and fluorometers. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R11490Quant-iT™ PicoGreen ® dsDNA Kit (Invitrogen, #P11496). 2 to 100 ng) Assay Kit were used with a Qubit 3. P7589. , to prepare enough working solution to assay 100 samples in 200 µL volumes, add 50 µL Quant-iT ™ RiboGreen RNA Reagent to 9. The final pooled sample was quantified using a Quant-IT PicoGreen dsDNA assay (Thermo Fisher Scientific) and the resulting fragment size distribution was assessed using a Bioanalyzer (Agilent. Lambda DNA was used to generate the. The PicoGreen reagent was freshly prepared by diluting 15 μL reagent with 2 mL of TE buffer (~ 133X) and stored in the dark for no longer than 2 hours. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. Quant-iT Assay Kits Quant-iT DNA Assay Kit, High Sensitivity The Quant-iT High-Sensitivity DNA Assay Kit has a linear detection range of 0. Figure 2. This protocol is a simplified and This protocol is a simplified and condensed version of the full protocol from the manufacturer. n = 3. Figure 8. 0). PicoGreen dsDNA quantitation reagents are. • Specific for dsDNA in the presence of RNA. Each Helixyte™ assay kit is specially formulated with a target-selective DNA. It requires the most user preparation and calculation, but can be adapted for cuvettes or plates. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. • Specific for dsDNA in the presence of RNA. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). Rapidly detect as little as 0. 005% Triton X-100, Tween-20, or Brij. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. Rapidly detect as little as 0. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT™ PicoGreen® dsDNA Assay Kit *10 x 100 µl* 1 kit P11496 Quant-iT™ PicoGreen® dsDNA Reagent 1 kit P7581 Quant-iT™ PicoGreen® dsDNA Reagent *10 x 100 µl* 1 kit P11495 Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 1 kit R11491 Quant-iT™ RiboGreen® RNA Assay Kit *2,000 assays* 1 kit R11490 Quant-iT™ OliGreen™ ssDNA. The PicoGreen assay has high specificity for dsDNA versus RNA or ssDNA, and is. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesQuant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Catalog number: P7581. i) 110 sample x 150uL =16. The high sensitivity kit is superior to the PicoGreen™ or Quant-iT™ dsDNA detection kits by having aPicogreen是一种极为灵敏的荧光核酸染料，其仅在与DNA双链结合后才发出荧光，且所产生的荧光与DNA浓度呈正比，因而可用于精确测量多个来源的dsDNA，包括基因组DNA、病毒DNA、PCR扩增产物、测序文库等。 本产品Picogreen dsDNA Quantitation Reagent定量具有以下优势：①灵敏度高：可检测低至200 pg的dsDNA，节省. Los ensayos Picogreen son compatibles con la mayoría de los lectores de microplacas y fluorómetros. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. 0 ml 200x in DMSO b. 2. Bioz Stars score: 86/100, based on 1 PubMed citations. Note: Allow the Quant-iT ™ RiboGreen RNA Reagent to warm to room temperature before. DNA. Prepared DNA concentration of the samples was 250 ng/ mL. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. 3,201 3. . Figure 1. Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R114909. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Picogreen assays are compatible with most microplate readers and fluorometers. (Left) The fluorescence signal from binding of the Quant-iT PicoGreen dsDNA Reagent to dsDNA is linear over at least four orders of magnitude; the inset shows the results obtained using 0–750 pg/ml calf thymus DNA. You will need 100 ul diluted PicoGreen per well containing 100 ul sample. Common contaminants such as salts, free nucleotides, solvents,Product Description. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 10pg/µl vs. • Specific for dsDNA in the presence of RNA. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. The method for this assay follows the instructions in the product information sheet for Quant-iT PicoGreen dsDNA Reagent and Kits from Molecular Probes, except that the assay volume is proportionately reduced from 2. Table 2 summarizes results of the. Rapidly detect as little as 0. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). The reagent Quant-iT PicoGreen was added to all wells and the samples were incubated for 5 min at room. • Suitable for use in 96- and 384-well plate formats. Rapidly detect as little as 0. The Quant-iT RNA Reagent and Assay Kit makes RNA quantification easy and accurate. Rapidly detect as little as 0. The fusion protein nsp7-nsp8 was generated by inserting a GSGSGS linker sequence. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 995 mL TE for the . coli rRNA (Δ), or a 1:1 mixture of DNA and RNA (∎) were assayed in the Quant-iTTM dsDNA HS assay. Our Quant-iT™ PicoGreen® dsDNA reagent enables researchers to quantitate as little as 25 pg/mL of dsDNA (50 pg dsDNA in a 2 mL assay volume) with a standard spectro fluoro- meter and. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 125 to 100 ng/mL. P11495. The standard PicoGreen assay protocol is also simpler than the Hoechst 33258 method, because a single concentration of the PicoGreen. 20 and 2. 3ng/mL，DNAThe fragment size of the amplicon was assessed using agarose gel electrophoresis and quantified by Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Further to the Quant-iT dsAssay kit (PicoGreen®), the Qubit dsDNA HS Assay kit offers an additional option for quantify-ing dsDNA samples of low concentration on the BioSpec-trometer fluorescence with high specificity and sensitivity. The fluorescence intensity as a function of the amount of dsDNA is measured at the optimal. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 7 Q33232 4 Green features Less waste and use of fewer resources With the Qubit and Quant-iT 1X dsDNA HS and 1X dsDNA BR Assay Kits, instead of supplying the assay reagent and dilution buffer in separate bottles as was done with the. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. Pico488 allows to quantitate as little as low pg/mL of dsDNA. PicoGreen® Assay Supplies Equipment: − NanoDrop 3300 Fluorospectrometer − 2uL pipettor (low retention nuclease free pipette tips) Materials:Total DNA recovery was determined with the Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Carlsbad, CA). The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. Rapidly detect as little as 0. Results: DNA concentration values were measured by the “DNA Mamm. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. 500/525. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. PicoGreen dsDNA quantitation reagents are. LC3, and dsDNA, or α-synuclein, cathepsin D and dsDNA, Picogreen (1/10000, Quant-iT PicoGreen dsDNA Assay Kit. Picogreen assays are compatible with most microplate readers and fluorometers. The assay is performed at room temperature, and the signal is stable for 3 hours. 0. • Suitable for use in 96- and 384-well plate formats. ITSkabir). Picogreen assays are compatible with most microplate readers and fluorometers. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. • Specific for dsDNA in the presence of RNA. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. BR reagent (Component A) 1. The Quant-iTTM PicoGreenTM dsDNA Reagent enables researchers to quantitate as little as 250 pg/mL of dsDNA (50 pg dsDNA in a 200 μL assay volume) with a fluorescence. The libraries were sequenced using the Illumina HiSeq 2500. ただ、この製品のマニュアルには蛍光分光光度計での測定方法しか. 蛍光を用いたDNAの定量法の一つとしてInvitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit が利用されています。. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 1 mL kit. When 5–500 pg salmon dsDNA or 3–1000 pg control dsDNA (calf thymus dsDNA standard) is added to AccuClear a linear response is observed (Fig. Rapidly detect as little as 0. The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL, providing a core detection range of 0. 04 corresponds to 2 μ g/mL dsDNA solution. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. • Specific for dsDNA in the presence of RNA. Rapidly detect as little as 0. This reagent is also included with the Quant-iT RiboGreen RNA Assay Kit and comes complete with buffer and RNA. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. The DNA sample used for aliquoting was extracted from soil using the DNeasy PowerSoil Kit (Qiagen). Double stranded DNA quantification performed in down to 2 µl using the LVis Plate. Picogreen assays are compatible with most microplate readers and fluorometers. The Quantus™ NGS Starter package is cost-effective and easy to incorporate into your laboratory. The limit of detection for the 20/20n is less than 45 pg in 100 µL total volume. Unit Size. 50 pg to 2 µg. Picogreen assays are compatible with most microplate readers and fluorometers. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%). The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. Contents and Storage Information. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT™ PicoGreen ® dsDNA Reagent (10 vials, each containing 100 µL aliquots) 25 mL of 20X TE buffer; 1 mL of 100 µg/mL Lambda DNA (dsDNA standard) These jellies cheapest cialis uk and pills are can be ingested with or without food. Picogreen Assay for DNA quantification: Which one is better to go for, a fresh. The total amount of DNA expected is in the range of 3–100 ng, with concentrations as low as 0. Price from $9. 2 to 100 ng) Assay Kit were used with a Qubit 3. Vortex solution briefly to mix well and centrifuge for 5 sec to bring liquid to bottom of tube; then dispense for use in the assay. P11495. The Quant-iT™ DNA Assays are designed for high throughput (>20 samples) and for use in 96-well plate readers with no further adaptation. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. Rapidly detect as little as 0. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. Module in combination with PicoGreen® dye allows the direct quantitation of dsDNA in as little as 100 µL total volume. Picogreen assays are compatible with most microplate readers and fluorometers. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 2mL 1 x 96 wellsAssay Plate with Plate Sealing Foil, D002 *Component can be purchased separately. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay for nucleic acid quantification. 作成者 LatB Staff 01. Sequencing was performed with a HiSeq Single-End Illumina platform (USA) [Citation 31]. Picogreen assays are compatible with most microplate readers and fluorometers. All NGS libraries generated with both the Fragmentase and the FTP method contained similar amounts of ds DNA (800 ± 50 ng) and had similar mean insert sizes of the libraries in a range from 400 to 500 bp. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. No. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5 microplate reader. Quant-iT dsDNA BR buffer (Component B) 250 ml c. Quant-iT™ PicoGreen ® dsDNA Kit (Invitrogen, #P11496). A Qubit dsDNA BR (broad range, 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Dilute from 20xTE stock (supplied in the PicoGreen kit). Not for use in diagnostic procedures. The PicoGreen dsDNA quantitation assay can be used when selectively detecting as little as 25 pg/ml of dsDNA with free nucleotides, RNA and ssDNA present, having minimal impact on the intensity of. Description. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. quant it picogreen dsdna assay kit reagents. 2. We quantified the extract with Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents (Thermos Fisher Scientific, Waltham, MA, USA) to evaluate the amount of residual DNA. Rapidly detect as little as 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. This protocol is a simplified and condensed version of. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. Determine the DNA concentration on the basis of absorbance at 260 nm (A 260) in a cuvette with a 1 cm pathlength; an A 260 of 0. to each well. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267DNA concentration was measured using a spectrometer with the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. The QuantiFluor® dsDNA Dye offers greater sensitivity over absorbance at 260nm (NanoDrop®) low-concentration samples, and better or equal sensitivity performance when compared to PicoGreen®. The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as. Product Type. The HS (high sensitivity) assays quantitate the lowest levels of analyte, the BR (broad range) assays do so across a wider range, and the RNA XR. After completion of DNA extraction, double-stranded DNA (dsDNA) is quantified by the Quant-iT™ PicoGreen® fluorescence assay using the provided lambda DNA standards (Invitrogen) prior to Library Construction (LC). Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. RCA products may be directly quantified using the Quant-iT® PicoGreen® dsDNA Assay Kit or with a Qubit® Fluorometer after dilution to the appropriate detection range. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. 99. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Determination of DNA Yield. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. All LNP samples were diluted 300-fold in TE buffer and placed on a Corning ® 96 Well Solid Polystyrene Microplate (Sigma-Aldrich, St. 0. Library quality and average fragment size was assessed using a Bioanalyzer (Agilent, G2939A) High Sensitivity DNA chip (Agilent, 5067-4626). Qubit assays are available for double- and single-stranded DNA (dsDNA and ssDNA), RNA, microRNA, and protein. Rapidly detect as little as 0. Quant-IT dsDNA Assay Kit, high sensitivity Quant-iT dsDNA Assay Kit, broad range Quant-iT 1X dsDNA high sensitivity; Target: dsDNA: dsDNA: dsDNA: Quantitation range: 0. Volume of 20xTE = final volume/20;Application tip demonstrating that with Molecular Devices SpectraMax® microplate textbooks and the Quant-iT PicoGreen assay, users can reliably measure contents as low when 50 pg/mL of double-stranded DNA. 2–100 ng, the fluorescence signal is linear with DNA. Rapidly detect as little as 0. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Spectrofluorometry was performed based on guidelines from ThermoFisher Scientific Inc. Silke Angersbach, Ron Earp, Mark Gröne, Franka Maurer, BMG LABTECH, 12/2014. After a 5-minute. This kit contains. Cloning, expression and proteins purification. Thermo Fisher Scientific公司的Quant-iT PicoGreen dsDNA 检测试剂盒对DNA更特异，比传统光吸收方法的灵敏度高约 1000倍。 如产品手册所述，此实验在微孔板格式中采用单 一染料浓度的动态范围从250 pg/mL到1000 ng/mL。在这 里，我们证明了使用Molecular Devices公司SpectraMax®Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. PicoGreen dsDNA quantitation reagents are. The tubes were. High linearity and sensitivity over a broad DNA range. • Suitable for use in 96- and 384-well plate formats.