SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Use similar settings for other Molecular Devices readers with fluorescence detection mode. SpectraMax L Microplate ReaderThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Compare plate readers The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The calculated LOQ values were 0. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. All . These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Devices’ StakMax® Microplate Stacker in a matter of minutes and . This is equivalent to having 511 built-in filters,The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. 76358-624. When the user applies a set temperature of 37°C, the top is heated to 37. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. 314 low lamp level warning. For more sample throughput, both readersThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 µL sample volume in a. Or, with the reader specifications noted from the Instrument Description section of your reader's. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The drawing shows a standard microplate of 15 mm height covered with a lid. Reporter Assay. Add to Cart. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. These lab workhorses are used in drug discovery, research, bioassay. For the GMP/GLP regulated labs, the systems come equipped with our SoftMax Pro GxP. SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate 312 gain calibration failed. Download PDF versions of the SpectraMax Microplate Reader User Guides. Table 1. flat meniscus), but is approx 15 mL. For readers without a cuvette port, simply check the box next to ‘PathCheck’. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. ATP standard curves read on SpectraMax microplate readers. 306 plate air cal. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. SpectraMax iD5 Multi-Mode Microplate Reader. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The 2-channel configuration is available with 0, 2, or 4 injectors. 1. Microplate Readers Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. Step 1: Turn on your microplate reader. The screw remains inside the instrument. The University of Rouen in Normandy uses the SpectraMax iD3 and. Download PDF. The SpectraMax Quant dsDNA Assay Kits consist of three products, each designed for a specific range of DNA concentrations and optimized for use with fluorescence microplate readers such as the SpectraMax microplate reader (Table 1). This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. . The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. . Figure 3. 0112-0174C 3 Contents SafetyInformation 4 Warnings,Cautions,Notes,andTips 4 Chapter1:Introduction 7 InstrumentFeatures 8 ComputerIntegration 9 PMTTechnology 9The eight-channel system consists of 8 sample beams and 8 reference beams, delivering both superior precision and speed of reading across the microplate. As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. The price range of a reader starts at 2. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. If it looks fine, then proceed. Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. 20 μL of Component A was added to each well of the microplate. Microplate Readers Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. Type: Clear: Add to Cart. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. SpectraMax Plus: SpectraMax Plus. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12The optical system of SpectraMax Plus384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 1000 nm). Figure 1. . Add to Cart. 100 fmol 384-well (75 µL) 0. In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. Narrow 2nm bandwidths deliver increased measurement accuracy and linearity over the widest range. SpectraMax 250 User. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. A preconfigured protocol is available in the software to. Introduction. Download PDF. Supplier: Molecular Devices. 1. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Step 4: Incubate at room temperature for 1 hour, protected from light. The screw remains inside the instrument. The drawing shows a standard microplate of 15 mm height covered with a lid. Avoid losing high signal. 303 unable to cal dark current. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. western blot membrane detection in a multimode microplate reader platform. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow toFlexStation® 3 Multi-Mode Microplate Reader. Nucleic Acid and Protein Quantitation. ATP standard curves read on SpectraMax microplate readers. The data sheet provides detailed information on the system's optical performance, data collection and analysis, plate stacker and robot integration, and validation options. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingevery Gemini EM Reader. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMinimum volume and maximum throughput with 96- or 384-well microplate compatibility. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Products : General, Microplate Readers. Results are shown in Table 1. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. 380 nm/Exc. See SpectraMax® Microplate Reader User Manual Download Page. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. Note that this procedure is only for the SpectraMax L model. Molecular Interactions. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Users can perform spectral scans from 250 nm to 750. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. 306 plate air cal fail,low light. 3) This warranty. MPN:SpectraMax Plus. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. Choose from a three- (M3), four- (M4), orIn this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. HTRF-compatible SpectraMax Multi-Mode Readers. Microplate Readers Biotek Epoch Microplate Reader. For a higher . Figure 1. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Gemini XPS Microplate Reader (or other SpectraMax reader with fluorescence detection mode) SoftMax Pro Software; Methods Set up the instrument and software. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). SpectraMax Glo Steady-Luc Reporter Assay Kit. ” Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Tecan Infinite Microplate Reader. 300 µL of Detergent Compatible Bradford reagent was added to each well. Three low volume 24-well bottom slides. Figure 2-23: Bottle holder To install the bottle holder: 1. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. This section contains the following topics:滤光片型光吸收微孔板读板机（酶标仪），简单易用，性价比高，检测结果精确. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. 0 out of 5 $ 9,995 $ 9,495. : 2 Oct 12 Page 6 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Read More. This technology allows for the automated regulation of well absorbance to a cuvette equivalent path length of 1 cm, which translates to. Temperature in the microplate chamber is isothermal, both at ambient and when the incubator is turned on. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. The Gemini XPS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. BENEFITS • No filters needed • Top- and bottom-read capability • Higher sensitivity • Validation tools • Robotics compatible SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual. The Molecular Devices SpectraMax XPS has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. SpectraMax ® M2 Multi-Mode Microplate Reader. 7°C (gradient heating design). The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell imaging, and fast kinetics. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. We would like to show you a description here but the site won’t allow us. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. 33 Dramatic aggregation of Alzheimer A b by Cu(II) is induced by conditions representing physiological acidosis . The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. 470 nm). Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. ; Page 2 2) All labor charges to repair the product for a period of one (1) year from the original date of delivery will be paid by Molecular Devices Corporation. In this product video, see how the SpectraMax M5 quickly reads samples. 7°C and the bottom to 36. Microplate Readers. A: Microplate Preparation. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Detailed specifications of each model reader are reported in Section. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Molecular Devices. Results are shown in Table 1. The SpectraMax 190 reader can be integrated with Molecular . Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. . Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. 230-1000 nm, Monochromator, ABS, FLR (top and bottom) and LUM, with incubation. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Other technologies such as imaging. This unlocks the transport slide. Analytical Technologies Group specializes in the maintenance and repair of Molecular Devices microplate readers, including the SpectraMax® and VersaMax™ absorbance readers. The plate was read on the SpectraMax Plus384 Absorbance Microplate Reader and SpectraMax i3x Multi-Mode Microplate Reader, using a preconfigured SpectraDrop protocol in SoftMax ® Pro Software. Molecular Devices: Molecular Devices. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. In addition, the SpectraMax® iD5 can be expanded to include bottom. 0 out of 5 $ 5,999. 2 software: see SoftMax® Pro 7. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. SpectraMax® Microplate Reader Suite of Products Knowledge Base Published Dec 16, 2020 | Updated Dec 06, 2021 Products : SpectraMax i3x reader,. 020 fmol 0. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. With optimized reagents and the industry-leading data acquisition and analysistool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to perfectly. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). 0 CART STOREVersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile microplate reader capable of quantitating samples in a variety of microplate formats. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. The plate reader can measure multiple readouts, including UV and visible absorbance. 1. The SpectraMax iD3 reader allows you to interact with your data any way you want, from anywhere you want. Cuvette Microplate wells. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Absorbance reading with SpectraMax® microplate readers and SoftMax® Pro Software enables optical density measurement of samples in microplate or cuvette format. limit of detection (LLD) for ATP was estimated for each microplate reader using the . Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. Maximum signal with lowest background. Data exemplifying linear ranges from the three different kits are presented in Figure 2. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today launched its. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. 0 out of 5 $ 23,995 $ 22,495. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. Most papers they cultured bacteria to OD600>0. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Fluorescence Microplate Readers. The Spectramax 250s xenon flash lamp and grating monochromator facilitates direct UV measurement of DNA, RNA, and protein in microplates. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax iD3 reader is fully compatible with the SpectraDropTM Micro Volume Microplate, enabling quantitation of precious low volume samples. We would like to show you a description here but the site won’t allow us. Lower limits of detection for ATP determined using the ATPliteAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. We would like to show you a description here but the site won’t allow us. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity. Italic and boldface type are used for emphasis. 40266 Email: drugdiscoverytech@lifetech. Read one sample or up to 384 in0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The Gemini EM can sample a variety of formats, from 6- to 384-well. The SpectraMax® ABS Plus Microplate Reader can run . 0 out of 5 $ 6,499. The following accessories are required: • Set of 2 Fluorescence Polarization Filters 485 nm BW 25 nm Polarized Vertical & HorizontalSpectraMax Microplate Readers. Dual-Glo luciferase assay system (Promega Cat. The chamber contains springs that position the cuvette in the proper alignment for a read. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. We would like to show you a description here but the site won’t allow us. . Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. 2. The advanced optical and. 1. Add to Cart. For readers without a cuvette port, these options are not shown in the software. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence. 5 12. 000 Euro/Dollars for a high-end multi-mode microplate reader with several detection modes and the best technology for highest sensitivity and fastest detection. . 20 μL of Component A was added to each well of the microplate. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Examples: “Press carefully to engage,” “ Do not. Prerequisites: SoftMax Pro 7. Other features and benefits include: Eliminate changing out filters. SpectraMax Mini User Guide. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. For readers without a cuvette port, simply check the box next to ‘PathCheck’. SoftMax® Pro 7. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. The SpectraMax® ABS Plus Microplate Reader can run . SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Step 2: Launch SoftMax Pro Software. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Example: [Drawer]. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. IMAP FP Settings in SoftMax ProSpectraMax M5 Multi-Mode Microplate Reader. Examples: “Press carefully to engage,” “ Do not. 6% for the on-board optics (monochromators). When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application. Multimode Microplate Reader. The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 1. Devices’ StakMax® Microplate Stacker in a matter of minutes and . It can run samples based on pre-defined protocols and standard filter modes, and is compatible with ELISA and protein quantification assays. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. 305 reference level saturation. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. microplate reader. 315 can't find zero order. Additionally, user-installable options allow the SpectraMax i3 System to. begin reading microplates with seven mouse clicks. The following document is intended to demonstrate setup of this instrument. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. 2 only: SpectraMax Mini Update. AlphaScreen. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3 allows users to customize workflow to perfectly. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Detailed specifications of each model reader are reported in Section 7, “Specifications”. More Product Information. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. High sensitivity is demonstrated with a tryptophan standard curve, and a shift in peak emission is shown using a lysozyme denaturation assay. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. In . The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. Sale. 1. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command. flexibility of monochromator-based systems. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. Fully tested with validations, warranty. Microplate Automation & Detection. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 72 MB. Microplate reader settings. The plate. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. It also offers a field-upgradeable cellular imaging option and a cooled PMT for low-light sensitivity. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Cell Analysis.