Scan every well . Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. *For Corning UV plates, mean values at 260 nm ranged from 0. † Bottom mounting for SpectraMax Paradigm Readers only. #i3X) with: SoftMax Pro 7. To ensure accurate cell numbers for the cell standard curves, cells were assayed immediately. Microplate format. 5 pM 2. The 384-well plate is limited to a maximum volume of a 30 µl addition in a much smaller diameter well, and using the 96. 8 mm distance between well bottoms to plate bottom; 50μL total well volume, with recommended working volume of 5-40μL; High base plate design is compatible with automation and for bar coding; Validated by Molecular Devices for the FLIPR reader. #PLUS 384) UV-transparent 96-well microplates (Corning cat. x. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. 0 (or higher) Software; Methods Data acquisition using the Workflow Editor. Microplates, cuvettes (via adapter) Detectors. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 1. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. QuickSync. Round well design with a clear flat bottom 2. 1 x plates. The opaque walls prevent stray light from being detected from neighbouring. This is equivalent to having 511 built-in filters, 384-well: 0. 6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. Microplates for applications that require high sample recovery rates. Integrated stacker option. The plate reader consisted of a large-area illumination unit and a parallel detection unit; see Fig. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. If the computer is connected to the Internet: Select Search Automatically for. This chapter is an introductory overview of the most commonly used assay methods to estimate the number of viable cells in multi-well plates. For biochemical assays, the washer removes unbound material and unreacted reagents. All other trademarks herein are the property of Molecular Devices, Inc. UV absorbance detection. Was the failure only of the Detection Estimate (Mag3) Test for the M3/M4/M5/M5e/Flex3?Gurard-Levin describes his lab’s microplate reader selection: “In our labs, specific and refined optics are important, as well as the ability to use high density plates including those with 384- or 1536-wells. This can increase throughput and efficiency, thus saving time and space in the lab. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Modefor the SpectraMax Multi-Mode Microp late Reader, as well as all other Molecular Devices' microplate readers. 8 mm distance between well bottoms to plate bottomValidated by Molecular Devices for the FLIPR reader. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. x. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. A Costar 24-well tissue culture plate with col1 = blanks and doubling cell5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12Reader Documentation Version No. …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. 6-well tissue-culture treated (TC) plate, sterile. 030 fmol Fluorescence Polarization (FP). SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. SpectraMax iD5. 384-Well SBS Microplate, clear, square well, flat bottom, sterile, with cover, working volume 90 µl, packaged in sleeves of 10. For more sample throughput, both readers can be easily. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). Instructions for adding new plates: CSI/CP2: Add plate. For more sample throughput, the system canThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 96- and 384-well. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. 384 well plate (Molecular Devices LLC) About; News;Disposable Pin Tool - 96 / 384 Head: Fixed Pin Tool - 384 Head: Grippable Pin Tool - 96 / 384 Head: Magnetic Pin Tool - 96 / 384 / 1536 Head: LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent: MICROPLATE CYTOMETRY: Nexcelom Bioscience: Celigo S Cell Imaging. (1536-well plate) 2 µL (quartz cuvette), 4 µL (disposable cuvette). Total volume (except deep well plates) is 120 mL. Detection cartridge modularity allows for easy addition of new read modes within minutes without. The FLIPR 384 system comes complete with 384. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. 2. 3. SLS & DLS: 2 µL quartz cuvettes 45 µL quartz cuvettes 4 mL generic glass cuvette DLS only:Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. 4 Equipment. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-wellThe FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The systems can handle any slide-based microarray study, from small one- or two-fluor applications to multiple-fluor high-throughput projects requiring automated. use as little as 2 µL samples with 24- or 64-well plates. U-bottom: 30-40 µl. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Dilution series for ZsGreen (green), AcGFP (blue) and DsRed (red) transfected cells. Outer wells aligned for proper viewing with FLIPR; Ideal for miniaturized 384 well fluorescent. Extended linear dynamic range. 76 ± 0. 2. Figure 2. SpectraMax i3x. The estimated lower limit of detection (LLD) in both 96- and 384-well plates is < 2 fg/well for firefly luciferase in the LMax II 384 and Analyst GT and 2–3 fg/ well in the SpectraMax M5. 340 PC384. . Dual injectors for kinetic assays. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices,. Features. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Packed 25 plates per sleeve, 2 sleeves per case. 5in (46. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andSpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. Molecular Devices deliver high throughput while showing good correlation with manual patch clamping data. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. X. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. x. 96-well fluorescent cell dilution series, top-read. Inquiry Basket. DynaPro ™ Plate Reader III. 00. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. 020 fmol 0. Learn how the PathCheck feature can be applied to protein quantitation experiments using Molecular Devices absorbance-based microplate readers. 96- and 384-well microplates. ". After 30 minutes the plate was read in Analyst GT with varying z heights. Washers configured for both 96- and 384-well plates. ,LTD (CAT#: STEM-S. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The plate was mixed gently for 2 minutes on a plate shaker and then allowed to incubate at room temperature for 10 minutes to stabilize luminescence. com Check our website for a current listing of worldwide distributors. 2. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. Absorbance cuvette port. 5–25 µl. #0200-7011) Instrument setupRound well design with a solid black flat bottom2. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . #R8190 (10) vials of component A*Instruments with a 96- or 384-well format will accommodate the most commonly used plates. 0 mP 96-well. Molecular Devices: n/a:. A thermal cycling reaction was immediately initiated. 2. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates andCompany Molecular Devices, LLC; Price Get Quote; Catalog Number CSI / CSI FL; Type Cell Imaging System; Measuring Time White light - 96-well/ 384 well microplate: <2 min Fluorescence - 96-well/ 384 well microplate: <8 min; Plate Formats 6, 12, 24, 96 & 384-well SBS microplates; Applications Cell Line Development, Fluorescence, Time-LapseNext-generation luminescence plate reader. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. e. -Flex3 link -we don't have this) complements the Tecans, in that it has an internal 8- or 16-tip pipetting head and performs multi-label measurements [fluorescence, fluorescence resonance energy transfer (FRET) and chemiluminescence] in real-time in 96 or 384 well format. For SpectraMax L or LMax II, LMax II 384 - no adapter used. Pfa1 and Pfa1 Gpx4-KO cells stably overexpressing murine FSP1 were seeded on separate 384-well plates (500 cells per well), and screened with a library of small-molecule inhibitor compounds, as. The assay methods covered include. The same measurement settings can be used for subsequent plates as long as the volumes, tracer and concentrations remain the same. Data for a 384-well plate can be collected in less than two minutes, as opposed to up to 30The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Fluorometers also vary in the size of samples they accept, with some using very small sample sizes to conserve precious sample materials (down to 0. For fluorescence intensity assays, the SpectraMax® M2 and M2e units feature an. 0. Molecular Devices Gemini XS Microplate Spectrofluorometer Details. 3% in white 96- and 384-well microplates Time. Incubate the plates at room temperature for 20 min before reading plate luminescence using a Paradigm plate reader (Molecular Devices) (see Note 14). ) 45 (W) x 70 (H) x 27 (D) Dimensions (cm) 114 (W) x 178. 8 mm distance between well bottoms to plate bottomValidated by Molecular Devices for the FLIPR reader. Recertification service. Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. 3. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 5 ng per well. com. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. SLS & DLS: 2 µL quartz cuvettes 45 µL quartz cuvettes 4 mL generic glass cuvette DLS only:Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. This integrated system provides users with a multi-detection for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. (40 µl maximum) Clear, white, or black. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. A2000 Full Wavelength Microplate Reader, 6 ~ 384 Well Plate, SHANGHAI PEIOU ANALYSIS INSTRUMENT CO. Overview. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the. 3 µl). Microplate is validated by molecular devices for the FLIPR reader. M2. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Molecular Devices makes. begin reading microplates with seven mouse clicks. For more sample throughput, the system canPage 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. 100 fmol 384-well (75 µL) 0. 0 OD for earlier models. Microplate readers provide the ideal measurement platform for the high-throughput. 2. Temperature Control: Ambient +5C up to 42C. Reads 96-well microplates in 5 seconds; Reads 384-well microplates in 16 seconds, Monochromator, tunable 1. For Research Use Only. and the A1 corner of the plate adapter (Figure 1-2). Versa Max. Each kit is sufficient. The plate was mixed gently for 2 minutes on a plate shaker and then allowed to incubate at room temperature for 10 minutes to stabilize luminescence. This chapter describes assays where data are recorded using a plate-reader; it does not cover assay methods designed for flow cytometry or high content imaging. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. Typically, the full spectrum of signal from. EMax® Plus Microplate Reader, Molecular Devices Catalog Number: (10119-370) Supplier: Molecular Devices Description: Robust, high value microplate reader designed to. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Ideal for miniaturized 384 well fluorescent. were pipetted into a 96-well black-walled μclear plate. 25 µL/well in a 384-well plate, using AlphaScreen Phosphotyrosine (PT66) Assay Kit. The user-friendly MultiWash™+ Microplate Washer from Molecular Devices, one of the pioneers in microplate detection, includes 20 preset protocols, works with both 96- and 384-well plates, and comes with 4 different wash/rinse bottles for out-of-the-box use. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Data for a 384-well plate can be collected in less than two minutes, as opposed to up to 30Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. We would like to show you a description here but the site won’t allow us. 2. Industry standard disposable (choose from recommended list). plates < 100 amol (384-well) 1 0200-7019. Figure 1. The patented Hybrid Technology offers flexibility and sensitivity across a broad range of applications. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. $542. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The SpectraMax Paradigm reader with HTRF Detection Cartridge offers high-throughput screening capability with excellent read times and Z’ factors. Shaking: Linear and Orbital. Four wash/rinse bottles are included and configurable for both 96- and 384. 0. Embla washers are durable and easy to maintain. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. This kit is designed for customers who. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. 2. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. Our microplate. Expand the Port (COM & LPT) Right click on the USB Serial Port (COMx) and select Properties. A ten-fold serial dilution of purified luciferase in PBS with 0. 1. Published Dec 16, 2020 | Updated Dec 16, 2020. Total volume (except deep. That explains the lower values on the LmaxII384 plate reader. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. 4 decades . The. Recertification service. The narrow bandwidths used by the model increase measurement accuracy and linearity over a wide. With optimized reagents, option to operate the readers. Artel MVS Bundle DM Std/800TSNB Reader Details. explore more. Two different agonists, acetylcholine and carbachol, were tested. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. If no reference reading is taken, the instrument uses the I 0 values stored in the NVRAM (non-volatile memory) of the. Industry standard disposable (choose from recommended list). X. VMax . 384-well format. for ELISAs and most cell-based assays. It has a. The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. VMax . It comes standard with 4 wash/rinse bottles and 1 waste bottle for maximum washing flexibility. 315 can't find zero order. Simultaneous dual emission detection enables read times of only 2:17 for an entire 384-well plate. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. For a higher . Dispense 10 µL of each ATP/ADP combination across an entire row of a 384-well plate. Sensitivity and dynamic range are demonstrated by the high Z’ factors obtained on the SpectraMax. Tb. 58% for the detection module and 2. 96-deep-round-well-plate, 384-deep-square-well-plate, 24-deep-well-plates. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 375 pM 1 FP Fluorescence Polarization detection for fluorescein- or rhodamine-like labels Using specific LED and EX/EM filters for 6- to 1536-well plates 0200-7009POS 0200-7010POS Fluorescein FP EX 485 nm, EM 535P and 535S nm Rhodamine FP EX 535 nm, EM 595P and 595S nm 96–1. SpectraMax ID5 Plate Reader (Molecular Devices). After incubation, the assay plate is transferred to the seal-peeler to remove the seal from the assay plate and transferred to washing. of samples in both 96- and 384-well microplates or in a cuvette at a selected wavelength for a single point in time (endpoint), over a specified period of time (kinetic), or over a selected wavelength range (spectral scan. x. 1. The plate reader can measure multiple readouts, including UV and. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices'. Not for use in diagnostic procedures. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. 1. SpectraMax® iD5 Multi-Mode Microplate Readers,6 to 384-Well Microplates, Molecular Devices, LLC (CAT#: STEM-LE-0504-LC) Product Keyword. the Molecular Devices SPECTRAmax® 190 and 250 plate. Plate Formats: 6- to 384-well plates. Supersedes 1. the use of a reader calibrated with primary NIST and NMI standards. FlexIII. 984. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. x. Alpha 384 HTS 96- and 384-well . Measure aggregation, aggregate size distributions, changes in solution molecular weight, and propensity for aggregation: indicators of both thermal stability (T onset, T m, T agg) and colloidal stability (A 2 /B 22,. 20 attomol ATP per well. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. Summary of Contents for Molecular Devices SpectraMax M2. x. 19 H mm that meet microplate industry standards. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. For nearly 30 years, Molecular Devices has provided scientists with . Infinite possibilities. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. x. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax Plus 384 Microplate Reader (Molecular Devices cat. x. Features. 6-384 (96- and 384-well with pipetting) 96-well microplates. The SpectraMax 190 reader can be integrated with Molecular . Analyst, M5, Flex 3, and SpectraMax L readers work differently. This reader provides visible absorbance, top-reading fluorescence intensity, and glow luminescence modes in 96- to 384-well plates with wavelength selection options from 340-650 nm. x. Description: The SpectraMax® L microplate reader measures flash and glow luminescence assays in both 96- and 384-well plates. San Jose, Calif. 1 OD at 230 nm. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. Determine concentration using the Beer-Lambert LawMolecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life. 012 fmol 0. Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. The color touch screen provides a visual user interface, making programming fast and intuitive. Outer wells aligned for proper viewing with FLIPRIdeal for miniaturized 384 well fluorescent homogeneous assaysNot treated (or medium binding)…Elisa Plate Readers and Microplate Readers at Biocompare. Important things to consider when purchasing a multimode microplate reader. 더 많은. Note: These settings are based on necessary cookies. Supplier: Molecular Devices. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Contact Us Phone: +1-800-635-5577 Web: Email: [email protected] nM Z Factor at EC. Add 100 µl (96-well plate) or 25 µl (384-well plate) of cell suspension to each well of poly-D-lysine coated plates. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today. Time-resolved phosphorescence. Application Area: Spectrophotometer for molecular biology. #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. VIS absorbance detection. FlexStation® 3 Multi-Mode MicroplateAlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. 48 ± 0. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 340 PC384. 00. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Time-resolved phosphorescence. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Gemini EM Reader scans. tools to expand the boundaries of their research. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. 9. In addition, SoftMax Pro SoftwareSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. All stand-alone instrument functions can be controlled using the software. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. 1536-well plates < 100 amol (384-well) 1 HTRF • Cisbio HTRF detection with optimized Xenon light source and . (1536-well plate) 2 µL (quartz cuvette), 4 µL (disposable cuvette). Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Streamline GLP/GMP compliance. Shaking: Linear, orbital, and double orbital. 0nm increments. These robust readers have been placed in labs from Antarctica to the. Gemini XPS Reader scans. M5. Fluorescence Microplate Reader Comparison Chart. Molecular Devices deliver high throughput while showing good correlation with manual patch clamping data. DynaPro ™ Plate Reader III. Uncontrolled Copy for Training and Reference Purposes Only . 48, 96, and 384 wells can be used in the SpectraMax M2 and SpectraMax M2e. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. x. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Find regional support. 384 well polystyrene microplates have plate dimensions of 127. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. MultiWash+ Microplate Washer. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Microplates for applications that require high sample recovery rates. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. The default pipettor dispensing speed is 50 µL/sec for 96-well plates and 20 µL/ sec for 384-well plates. The FLIPR 384 system comes complete with 384. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. Top. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. Unparalleled performance on a personalized platform. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. To address this concern, Molecular Devices offers the FlexStation® 3 Multi-Mode Microplate Reader. x. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. Fluid dispensing speed. The modular platform allows upgrading to expand functionality as your laboratory workflows change. San Jose, CA 95134. Next generation SpectraMax® absorbance microplate readers. Figure 1. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to calculate the. With the FLIPR 384 , you get the sensitivity, data fidelity, and real-time, kinetic information of the FLIPR I system in a 384-well mode. x. 3860 N First Street. for the SpectraMax Multi-Mode Microp late Reader, as well as all other Molecular Devices' microplate readers. Next-generation luminescence plate reader. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Read one sample or up to 384 samples in a single plate by using any standard cuvette, or 96- or 384-well microplate. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. SpectraMax® ABS Plus microplate reader can run both cuvette-based and microplate reader applications on the same instrument. In addition, SoftMax Pro Software After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. This unlocks the transport slide.